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Peasy-t5 zero cloning kit

Webrecovered using a DNA recovery kit (Tiangen Biotech Co., Ltd, Beijing, China). The recovered PCR products were cloned using the pEASY-T5 Zero Cloning Kit (TransGen Biotech Co., Ltd, Beijing, China) by following the product manual. Two positive final clones were submitted to Invitrogen Corporation (Guangzhou, China) for sequencing. WebProduct Details. pEASY® -Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection.

pEASY® -T5 Zero Cloning Kit - TransGen Biotech

WebJul 11, 2024 · For the sensitivity detection, plasmid pEASY-T5-MDV which contains a 407 bp target segment in the pEASY™-T5 Zero Cloning Vector was constructed and sequenced. The purified positive control pEASY-T5-MDV plasmid (4362 bp) was used as the initial template with a DNA concentration of 31.7 ng/μL. The initial copy number was about 6.7× … WebMay 28, 2024 · The products were ligated to T5 zero cloning vector and transformed with Trans1-T1 into Competent cells by the heat-shock process at 42°C for 30 s (pEASY®-T5 Zero Cloning Kit). Three colonies were randomly picked from each transformation and incubated in Luria-Bertani liquid media at 37°C overnight under continuous shaking. One … spanish unique baby boy names https://lumedscience.com

Cloning and Mutagenesis System Supplier - Gaia Science Singapore

WebThe optimal vector-to-insert molar ratio is 1:7. · It can be roughly calculated at the ratio of "1 kb- to-20 ng" (e. g. 20ng for 1 kb inserted DNA, ; 30 ng for 1.5 kb inserted DNA) (The amount can be quantified roughly by TransGen's DNA molecular weight standard on the gel.) · The maximum amount of fragment addition is 4 μl, and do not ... WebEvaluation of pEASY- Uni Seamless Cloning and Assembly Kit to clone multiple fragments of Elaeis guineensis DNA. ... The pEASY™-T5 Zero Cloning Vector (TransGen, Beijing, China) was used for DNA ... WebJan 8, 2016 · The recovered PCR products were cloned using the pEASY-T5 Zero Cloning Kit (TransGen Biotech Co., Ltd, Beijing, China) by following the product manual. Two positive final clones were submitted to Invitrogen Corporation (Guangzhou, China) for sequencing. Prokaryotic Expression and SDS-PAGE Analysis. spanish upside down exclamation point alt

CT501-01 pEASY®-T5 Zero Cloning Kit Clinisciences

Category:Cloning and allelic variation of two novel catalase genes …

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Peasy-t5 zero cloning kit

Subcloning of one insert into a yeast expression vector pWXY1.0 …

Web关于其他常用耗材的网上超市合同公告采购信息，招标信息，机电设备采购平台 WebOct 4, 2024 · Each PCR product was inserted into the pEASY-T5 Zero Cloning Vector (TransGen Biotech, Beijing), and more than five clones per reaction were sequenced (Ykang, Hangzhou, China). The 5’ and 3’RACE fragments amplified using the corresponding primers were then assembled using DNAMAN 8.0 software (Lynnon Biosoft, Canada).

Peasy-t5 zero cloning kit

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WebpEASY-Blunt Zero (linearized) Cloning vector for blunt-ended PCR products, with a lacZα-ccdB suicide gene that allows for selection of recombinant plasmids. Sequence Author: … WebLinearized version of pUC19 for use with the GeneArt® Seamless Cloning and Assembly Kit. Linearized version of pUC19 for use with the GeneArt® Seamless Cloning and Assembly Kit. Features; Plasmids; ... pEASY-T5 Zero (linearized) pKF 18k-2. pSMART LCKan (linearized) CMV promoter. pEX-1. pKF 19k-2. pSP64 Poly(A) GAL1 promoter. pEX-A. pKOV ...

WebFree Trial pEASY-Blunt Simple (linearized) Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a T7 promoter but … WebThe Zero Blunt™ PCR Cloning Kit uses the multipurpose cloning vector pCR™-Blunt and ExpressLink™ T4 DNA Ligase to generate a ligation product in a five-minute, room-temperature ligation step. The Zero Blunt™ PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants ...

WebFeatures of the pEASY-Blunt Zero Cloning Kit. 5 minutes fast insertion of blunt-end PCR products.by topoisomerase. High cloning efficiency. Positive clones up to 100%. No blue/white selection needed. Suitable for larger … WebpEASY® -T5 Zero Cloning Kit Catalog Number：CT501-01 Price：$112.00 Specification： 20 rxns 60 rxns Quantity： Manual Vector Information MSDS COA Inquire Now Buy Now …

WebJan 22, 2024 · The product was cloned into the pEASY-T5 Zero Cloning vector (TransGene Biotech, Beijing, China) for sequencing. For 5′ RACE, the gene specific primers were designed based on the 3′ cDNA end sequence. The 5′ RACE cDNA template was synthesized by SMARTer@RACE cDNA Amplification Kit (TaKaRa, Tokyo, Japan).

WebThe recovered PCR products were cloned using the pEASY-T5 Zero Cloning Kit (TransGen Biotech (Beijing) Co., Ltd, Beijing, China) by following the product manual. Two positive final clones were submitted to Invitrogen Corporation (Guangzhou, China) for sequencing. Taq 2X PCR Master Mix, DNA Marker, deoxyribonucleoside triphosphates (dNTPs) and ... spanish university rankingsWebOct 1, 2016 · pEASY®-T5 Zero Cloning Kit – CT501. $ 338.00 – $ 673.00 CAD. 0 in stock (expect 7-21 days for delivery) Format. You will earn 16$ if you order this product! $ … spanish unoWebpEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed … tea tree oil for hair regrowthWebpEASY-T5 Zero Cloning Kit (TransGen Biotech, Beijing, China). Both of the recombinant plasmids were verified by sequencing. All the sequences of the primers used in this study are shown in spanish unisex namesWeb产品详情介绍. 该载体通过自杀基因表达与否筛选阳性重组子。. 当载体与片段连接成功时，自杀基因无法正确表达，包含重组子的细胞可以正常生长；当载体与片段连接不成功时，自杀基因正确表达，包含载体的细胞无法生长，即“Zero”背景。. 适用于TA克隆 ... tea tree oil for gum inflammationWebpEASY cloning kits a proprietary topoisomerase (TOPO) enzyme blend to efficiently clone your DNA in any of the pEASY vectors in only 5 minutes. ... pEASY-Blunt Zero Cloning Kit – CB501 $ 338.00 – $ 898.00 CAD; pEASY-Blunt3 Cloning Kit – CB301 ... pEASY®-T5 Zero Cloning Kit – CT501 $ 338.00 – $ 673.00 CAD; Filter by Supplier. Most ... spanish urban legend gold coinsWebFeb 15, 2024 · 分别以杜梨cDNA和gDNA为模板扩增PbGID1家族基因。扩增程序为：95 ℃预变性2 min；95 ℃ 30 s，55 ℃ 30 s，72 ℃ 1 min，30个循环；72 ℃ 延伸 2 min。PCR产物进行胶回收后，连接至pEASY-Blunt Zero Cloning Kit克隆载体，转化大肠杆菌感受态DH5α中，挑取单克隆测序。 spanish uom