Mitotracker red staining protocol
WebThese Mitotracker dyes are cell-permeant mitochondrion-selective dyes. These dyes are consistent, reliable, and stable, allowing for the generation of high-quality data. These … Web29 sep. 2024 · To further investigate the effect of CCG-1423 on mitochondrial morphology and membrane potential, the MitoTracker staining was ... for 5 min with DAPI (1:1000) to label the nuclei (ThermoFisher, Waltham, MA, USA, D1306). Cells were stained with MitoTracker™ Red ... E. High-resolution respirometry: OXPHOS protocols for human ...
Mitotracker red staining protocol
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WebFluorescence staining protocol was described previously (7. Larson-Casey J.L. Vaid M. Gu L. He C. Cai G.Q. Ding Q. Davis D. Berryhill T ... DAPI (MP Biologicals; 157577) is used for nuclear staining for 10 mins. Mitotracker red is used for mitochondrial staining for 20 min. Cells were fixed with cover slide using ProLong™ Gold Antifade ... Web11 apr. 2024 · MitoTracker red CMXRos/annexin V FITC staining. To assess the apoptosis of KGN cells, the MitoTracker red CMXRos/Annexin V FITC staining kit (Beyotime, C1035) was used according to the manufacturer’s instructions. Briefly, at the end of the experiments, KGN cells were harvested and stained with MitoTracker CMXRos and Annexin V FITC …
WebMitotracker is provided in 50 μg tubes and should be stored in the dark at -20 o C, indefinitely; it is stable in solution for up to 1 month, though we have not tested its … WebMitoTracker ® dyes are cell permeable probes that contain a mildly thiol-reactive chloromethyl moiety for mitochondrial labeling. To label mitochondria, cells are simply incubated with MitoTracker ® probes, …
WebTbSIR2rp3 GFP (green) localises up and mitochondrion (red; stained with Mitotracker) in bloodstream form T. brucei; DAPI smearing reveals the nucleus both kinetoplast (large and small select stain, respectively); scale bar, 5 µm. … Web12 apr. 2024 · For mitochondrial and lysosomal motility 25 nM MitoTracker Red CMXRos (Invitrogen, #M7512), and 25 nM LysoTracker red (Invitrogen, #L7528) ... Fixation and staining protocols were performed as described previously. 23 Cells were incubated with primary antibodies for 1 h at room temperature.
Web21 dec. 2024 · MitoTracker® staining is brighter in CD4 + T cells than CD8 + T cells. (A) MT deep red and MT green staining was compared between CD4 + and CD8 + T cells …
Webwill inhibit staining. Excitation: 490 nm Emission: 516 nm Background: MitoTracker® dyes are cell permeable probes that contain a mildly thiol-reactive chloromethyl moiety for mitochondrial labeling. To label mitochondria, cells are simply incubated with MitoTracker® probes, which passively diffuse across the plasma membrane and accumulate in sentara leigh hospital npiWeb28 mei 2024 · Here, we show how flow cytometry can be used to track changes in mitochondrial mass, membrane potential and superoxide (ROS) production in live … sentara leigh hospital central schedulingWebTo overcome this limitation, Molecular Probes has developed MitoTracker probes ― a series of patented mitochondrion-selective stains that are concentrated by active mitochondria and well retained during cell fixation. 16 Because the MitoTracker Orange, MitoTracker Red and MitoTracker Deep Red probes are also retained following … sentara leigh hospital central testingWebIn live tissue MitoTracker ® Red CMXRos and IraZolve-Mito staining and NAD(P)H detection should produce this characteristic mitochondrial staining pattern. The emission intensity from IraZolve-Mito should not decrease over time in tissue samples, and the protocol can be used for prolonged imaging at normal light intensity with minimal … sentara leigh hospital edWeb11 apr. 2024 · Confocal images of H9C2 stained with MitoTracker® Red representing the mitochondrial morphology and the mean mitochondrial area. Data were expressed as mean ± SEM (where n = 4). Data were analyzed using ordinary one-way ANOVA followed by Dunnet's multiple comparison test with a single pooled variance, where **P < 0.01, Vs … sentara leigh hospital jobsWebContribute to carpenter-singh-lab/2024_Cimini_NatureProtocols development by creating an account on GitHub. sentara leigh hospital emergency departmentWebMitoTracker 染料具有多种波长选择，可用于多色分析实验中的线粒体定位。 细胞固定后的染料保留情况：MitoTracker Deep Red 等染料固定后可充分保留，MitoTracker Red 则会固定后丢失。 请根据如下选择指南查找适合您实验的染料。 选择 MitoTracker 标记探针 固定细胞线粒体染色的偶联物 使用抗线粒体蛋白的荧光抗体偶联物，可标记固定细胞中的线 … sentara leigh hospital walk in clinic